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expression vector ppiczαa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher expression vector ppiczαa
    Expression Vector Ppiczαa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppiczalphaa+vector/pmc11724913-176-6-9
    Average 90 stars, based on 1 article reviews
    expression vector ppiczαa - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Virus:

    Article Title: Thermodynamic and structural description of allosterically regulated VEGFR-2 dimerization.
    Article Snippet: .. Human VEGF-A121, VEGF-A165, and VEGF-C and pox virus VEGF-E NZ2 with an aminoterminal hexahistidine tag were cloned into the pPICZAlphaA vector (Invitrogen), and the proteins were expressed in Pichia pastoris, as described previously.22 Culture supernatants were concentrated and dialyzed against 50mM Tris-HCl (pH 8.0) and 300mM NaCl and the proteins further purified by immobilized metal affinity chromatography (IMAC) using a Ni2 -charged chelating Sepharose column (GE Healthcare), and eluted with an imidazole gradient from 0-500mM. .. The proteins were deglycosylated with Endoglycosidase F1 (Endo F1) and further purified by size-exclusion chromatography (SEC) on Superdex 200 (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5), 150mM NaCl, and 5% (vol/vol) glycerol.

    Clone Assay:

    Article Title: Thermodynamic and structural description of allosterically regulated VEGFR-2 dimerization.
    Article Snippet: .. Human VEGF-A121, VEGF-A165, and VEGF-C and pox virus VEGF-E NZ2 with an aminoterminal hexahistidine tag were cloned into the pPICZAlphaA vector (Invitrogen), and the proteins were expressed in Pichia pastoris, as described previously.22 Culture supernatants were concentrated and dialyzed against 50mM Tris-HCl (pH 8.0) and 300mM NaCl and the proteins further purified by immobilized metal affinity chromatography (IMAC) using a Ni2 -charged chelating Sepharose column (GE Healthcare), and eluted with an imidazole gradient from 0-500mM. .. The proteins were deglycosylated with Endoglycosidase F1 (Endo F1) and further purified by size-exclusion chromatography (SEC) on Superdex 200 (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5), 150mM NaCl, and 5% (vol/vol) glycerol.

    Article Title: Structural analysis of vascular endothelial growth factor receptor-2/ligand complexes by small-angle X-ray solution scattering.
    Article Snippet: Receptor tyrosine kinases play essential roles in tissue development and homeostasis, and aberrant signaling by these molecules is the basis of many diseases.. Understanding the activation mechanism of these receptors is thus of high clinical relevance.. We investigated vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs), which regulate blood and lymph vessel formation.

    Plasmid Preparation:

    Article Title: Thermodynamic and structural description of allosterically regulated VEGFR-2 dimerization.
    Article Snippet: .. Human VEGF-A121, VEGF-A165, and VEGF-C and pox virus VEGF-E NZ2 with an aminoterminal hexahistidine tag were cloned into the pPICZAlphaA vector (Invitrogen), and the proteins were expressed in Pichia pastoris, as described previously.22 Culture supernatants were concentrated and dialyzed against 50mM Tris-HCl (pH 8.0) and 300mM NaCl and the proteins further purified by immobilized metal affinity chromatography (IMAC) using a Ni2 -charged chelating Sepharose column (GE Healthcare), and eluted with an imidazole gradient from 0-500mM. .. The proteins were deglycosylated with Endoglycosidase F1 (Endo F1) and further purified by size-exclusion chromatography (SEC) on Superdex 200 (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5), 150mM NaCl, and 5% (vol/vol) glycerol.

    Article Title: Candida albicans β-1,2-mannosyltransferase Bmt3 prompts the elongation of the cell-wall phosphopeptidomannan.
    Article Snippet: .. The 1401 bp long DNA fragment was then digested with PmlI and NotI, and ligated into the PmlI/NotI sites of the pPICZalphaA vector (Life Technologies). ..

    Purification:

    Article Title: Thermodynamic and structural description of allosterically regulated VEGFR-2 dimerization.
    Article Snippet: .. Human VEGF-A121, VEGF-A165, and VEGF-C and pox virus VEGF-E NZ2 with an aminoterminal hexahistidine tag were cloned into the pPICZAlphaA vector (Invitrogen), and the proteins were expressed in Pichia pastoris, as described previously.22 Culture supernatants were concentrated and dialyzed against 50mM Tris-HCl (pH 8.0) and 300mM NaCl and the proteins further purified by immobilized metal affinity chromatography (IMAC) using a Ni2 -charged chelating Sepharose column (GE Healthcare), and eluted with an imidazole gradient from 0-500mM. .. The proteins were deglycosylated with Endoglycosidase F1 (Endo F1) and further purified by size-exclusion chromatography (SEC) on Superdex 200 (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5), 150mM NaCl, and 5% (vol/vol) glycerol.

    Affinity Chromatography:

    Article Title: Thermodynamic and structural description of allosterically regulated VEGFR-2 dimerization.
    Article Snippet: .. Human VEGF-A121, VEGF-A165, and VEGF-C and pox virus VEGF-E NZ2 with an aminoterminal hexahistidine tag were cloned into the pPICZAlphaA vector (Invitrogen), and the proteins were expressed in Pichia pastoris, as described previously.22 Culture supernatants were concentrated and dialyzed against 50mM Tris-HCl (pH 8.0) and 300mM NaCl and the proteins further purified by immobilized metal affinity chromatography (IMAC) using a Ni2 -charged chelating Sepharose column (GE Healthcare), and eluted with an imidazole gradient from 0-500mM. .. The proteins were deglycosylated with Endoglycosidase F1 (Endo F1) and further purified by size-exclusion chromatography (SEC) on Superdex 200 (GE Healthcare) equilibrated with 50mM HEPES (pH 7.5), 150mM NaCl, and 5% (vol/vol) glycerol.

    Construct:

    Article Title: Core-glycosylated HCV envelope proteins
    Article Snippet: .. The shuttle vector pPICZalphaE′E1sH6 was constructed starting from the pPICZalphaA vector (Invitrogen; SEQ ID NO:51, FIG. 29). ..

    Sequencing:

    Article Title: Structural analysis of vascular endothelial growth factor receptor-2/ligand complexes by small-angle X-ray solution scattering.
    Article Snippet: Receptor tyrosine kinases play essential roles in tissue development and homeostasis, and aberrant signaling by these molecules is the basis of many diseases.. Understanding the activation mechanism of these receptors is thus of high clinical relevance.. We investigated vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs), which regulate blood and lymph vessel formation.



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